NSJ Bioreagents

PRDX1 Antibody (Peroxiredoxin 1)

Product Code:
 
NSJ-R31761
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
The PRDX1 antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of PRDX1 antibody and Lane 1: rat brain; 2: mouse brain; 3: human U87; 4: (m) Neuro-2a; 5: (h) A375; 6: (h) 293T; 7: (h) SMMC; 8: (h) A549; 9: (h) RH35 lysate. Expected size: ~22KD
2 / 6
IHC-P testing of PRDX1 antibody and human breast cancer tissue. HIER: steamed with pH6 citrate buffer.
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ICC testing of PRDX1 antibody and (h) SMMC-7721 cells
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IHC-P testing of mouse brain tissue. HIER: steamed with pH6 citrate buffer.
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IHC-P testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
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IF/ICC staining of FFPE human HeLa cells with PRDX1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of PRDX1 antibody and Lane 1: rat brain; 2: mouse brain; 3: human U87; 4: (m) Neuro-2a; 5: (h) A375; 6: (h) 293T; 7: (h) SMMC; 8: (h) A549; 9: (h) RH35 lysate. Expected size: ~22KD
IHC-P testing of PRDX1 antibody and human breast cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of PRDX1 antibody and (h) SMMC-7721 cells
IHC-P testing of mouse brain tissue. HIER: steamed with pH6 citrate buffer.
IHC-P testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
IF/ICC staining of FFPE human HeLa cells with PRDX1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

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CodeSizePrice
NSJ-R31761-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
Titration of the PRDX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Peroxiredoxin 1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes which reduce hydrogen peroxide and alkyl hydroperoxides.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
5052
Immunogen:
An amino acid sequence from the middle region of human Peroxiredoxin 1 (MNIPLVSDPKRTIAQDYGVLKADEGISFR) was used as the immunogen for this PRDX1 antibody.
Limitation:
This Peroxiredoxin 1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat