NSJ Bioreagents

PRDM1 Antibody / BLIMP1

Product Code:
 
NSJ-RQ4227
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
After reconstitution, the BLIMP1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 8
Western blot testing of human 1) HeLa, 2) A549, 3) 293T, 4) MCF7 and 5) 22RV1 cell lysate with PRDM1 / BLIMP1 antibody at 0.5ug/ml. Predicted molecular weight: ~92 kDa.
2 / 8
Western blot testing of rat 1) thymus, 2) spleen, 3) stomach, 4) lung and mouse 5) thymus, 6) spleen, 7) stomach, 8) lung and 9) HEPA1-6 lysate with BLIMP1 antibody at 0.5ug/ml. Predicted molecular weight: ~92 kDa.
3 / 8
IHC testing of FFPE human breast cancer tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 8
IHC testing of FFPE human colon cancer tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 8
IHC testing of FFPE human lung cancer tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
6 / 8
IHC testing of FFPE human placental tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
7 / 8
IHC testing of FFPE mouse small intestine tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
8 / 8
IHC testing of FFPE rat small intestine tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
❮❯

Western blot testing of human 1) HeLa, 2) A549, 3) 293T, 4) MCF7 and 5) 22RV1 cell lysate with PRDM1 / BLIMP1 antibody at 0.5ug/ml. Predicted molecular weight: ~92 kDa.
Western blot testing of rat 1) thymus, 2) spleen, 3) stomach, 4) lung and mouse 5) thymus, 6) spleen, 7) stomach, 8) lung and 9) HEPA1-6 lysate with BLIMP1 antibody at 0.5ug/ml. Predicted molecular weight: ~92 kDa.
IHC testing of FFPE human breast cancer tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human colon cancer tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human lung cancer tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human placental tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse small intestine tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat small intestine tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-RQ4227-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the BLIMP1 antibody should be determined by the researcher.
Description:
PR domain zinc finger protein 1, also known as B Lymphocyte Induced Maturation Protein 1 (BLIMP-1), is a protein that in humans is encoded by the PRDM1 gene. This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids M1-Q230 was used as the immunogen for the PRDM1 / BLIMP1 antibody.
Limitation:
This BLIMP1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O75626