NSJ Bioreagents

Nucb2 Antibody / Nucleobindin 2

Product Code:
 
NSJ-R32171
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Nucb2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 3
Western blot testing of mouse HEPA cell lysate with Nucb2 antibody. Predicted molecular weight ~50 kDa.
2 / 3
IHC testing of FFPE mouse stomach with Nucb2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 3
IHC testing of FFPE rat stomach with Nucb2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

Western blot testing of mouse HEPA cell lysate with Nucb2 antibody. Predicted molecular weight ~50 kDa.
IHC testing of FFPE mouse stomach with Nucb2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat stomach with Nucb2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

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NSJ-R32171-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Nucb2 antibody should be determined by the researcher.
Description:
Nucleobindin-2 is a calcium-binding EF-hand protein that in humans is encoded by the NUCB2 gene. By exon trapping and sequence analysis, the NUCB2 sequence has been identified on chromosome 11p15-p14. This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 25-106 of mouse Nucleobindin 2 were used as the immunogen for the Nucb2 antibody.
Limitation:
This Nucb2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
P81117