NSJ Bioreagents

NRF1 Antibody

Product Code:
 
NSJ-R31050
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NRF1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 5
Western blot testing of human 1) U-2 OS and 2) HeLa cell lysate with NRF1 antibody.
2 / 5
IHC-P: NRF1 antibody testing of human breast cancer tissue
3 / 5
IHC-P: NRF1 antibody testing of human intestinal cancer tissue
4 / 5
ICC testing of NRF1 antibody and A549 cells
5 / 5
IHC-P: NRF1 antibody testing of rat intestine tissue
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Western blot testing of human 1) U-2 OS and 2) HeLa cell lysate with NRF1 antibody.
IHC-P: NRF1 antibody testing of human breast cancer tissue
IHC-P: NRF1 antibody testing of human intestinal cancer tissue
ICC testing of NRF1 antibody and A549 cells
IHC-P: NRF1 antibody testing of rat intestine tissue

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CodeSizePrice
NSJ-R31050-100ug100 ug£535.00
Quantity:
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the NRF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Nuclear Respiratory Factor 1 is also known as Alpha-Pal. Efiok et al.(1994) identified genes containing NRF1-binding sequences and found that these could be classified either as cellular proliferation genes, or as genes regulating the growth-responsive metabolic pathways of energy transduction, translation, and replication. Virbasius and Scarpulla(1994) noted that the nuclear-encoded mitochondrial transcription factorTFAMcontains potential binding sites for NRF1, NRF2(GABPA) and SP1 within the promoter region. With use of binding and electrophoretic mobility shift assays, DNase footprinting, and mutation analysis of recombinant proteins, they demonstrated specific and functional binding ofNRF1andNRF2to the TFAM promoter region.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human Nuclear Respiratory Factor 1 (QHGREDLLYAFEDQQTQ) was used as the immunogen for this NRF1 antibody (100% homologous in human, mouse and rat).
Limitation:
This NRF1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q16656