NSJ Bioreagents

NOX1 Antibody

Product Code:
 
NSJ-R30751
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NOX1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 2
IHC staining of FFPE rat colon tissue with NOX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.~
2 / 2
Western blot testing of 1) rat brain and 2) mouse brain tissue with NOX1 antibody. Predicted molecular w
❮❯

IHC staining of FFPE rat colon tissue with NOX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.~
Western blot testing of 1) rat brain and 2) mouse brain tissue with NOX1 antibody. Predicted molecular w

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NSJ-R30751-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the NOX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NADPH OXIDASE 1, also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. It is expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of NOX1 in NIH3T3 cells increased superoxide generation and cell growth. Cells expressing NOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of rat NOX1 (WYKFQRAHNKLKTQK) was used as the immunogen for this NOX1 antibody.
Limitation:
This NOX1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
Q9WV87