NSJ Bioreagents

Nkx2-5 Antibody

Product Code:
 
NSJ-R31706
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the Nkx2-5 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of Nkx2-5 antibody and Lane 1: mouse spleen; 2: mouse heart; 3: human HeLa lysate. Predicted/observed size ~35KD
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Western blot testing of Nkx2-5 antibody and Lane 1: mouse spleen; 2: mouse heart; 3: human HeLa lysate. Predicted/observed size ~35KD

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NSJ-R31706-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Nkx2-5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Homeobox protein Nkx-2.5, also known as NKX2E or CSX, is a protein that in humans is encoded by the NKX2-5 gene. It is mapped to 5q35.1. Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. Nkx2-5 and Tbx5 directly bound to the promoter of the gene encoding cardiac-specific natriuretic peptide precursor type A (NPPA) in tandem, and both transcription factors showed synergistic activation. Nkx2-5 is essential in cardiac development, and mutations in the protein cause various congenital heart malformations.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
1482
Immunogen:
An amino acid sequence from the middle region of human Homeobox protein Nkx-2.5 (AKDPRAEKKELCALQKAVELEKTEADNAER) was used as the immunogen for this Nkx2-5 antibody.
Limitation:
This Nkx2-5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse