NSJ Bioreagents

NFAT2 Antibody / NFATC1 (isoforms A/B/C)

Product Code:
 
NSJ-R32829
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NFAT2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 5
Western blot testing of 1) mouse thymus and 2) human 22RV1 lysate with NFAT2 antibody at 0.5ug/ml. Predicted molecular weight: ~77 kDa (isoform A), ~88 kDa (isoform B), ~101 kDa (isoform C).
2 / 5
Flow cytometry testing of human THP-1 cells with NFAT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT2 antibody.
3 / 5
Flow cytometry testing of human MCF-7 cells with NFAT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT2 antibody.
4 / 5
Flow cytometry testing of human HeLa cells with NFAT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT2 antibody.
5 / 5
Flow cytometry testing of human Jurkat cells with NFAT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT2 antibody.
❮❯

Western blot testing of 1) mouse thymus and 2) human 22RV1 lysate with NFAT2 antibody at 0.5ug/ml. Predicted molecular weight: ~77 kDa (isoform A), ~88 kDa (isoform B), ~101 kDa (isoform C).
Flow cytometry testing of human THP-1 cells with NFAT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT2 antibody.
Flow cytometry testing of human MCF-7 cells with NFAT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT2 antibody.
Flow cytometry testing of human HeLa cells with NFAT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT2 antibody.
Flow cytometry testing of human Jurkat cells with NFAT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=NFAT2 antibody.

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NSJ-R32829-100ug100 ug£535.00
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Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA (human recombinant protein: 0.1-0.5ug/ml (BSA-free formulation available)
Application Note:
Optimal dilution of the NFAT2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
NFATC1 (Nuclear factor of activated T-cells, cytoplasmic 1), also known as NFATC or NFAT2, is a protein that in humans is encoded by the NFATC1 gene. And it is also a component of the nuclear factor of activated T cells DNA-binding transcription complex. The NFATC1 gene is mapped on 18q23. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. NFATC1 was not detected in brain, liver, or kidney. NFATC1 is an inducible nuclear component which functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. The solution structure of the binary complex formed between the core DNA-binding domain of human NFATC1 and the 12-bp oligonucleotide duplex containing the ARRE2 DNA site from the IL2 promoter. Nfatc1 was expressed exclusively in mouse hair follicle stem cells, and using gain- and loss-of-function approaches, and Nfatc1 inhibited stem cell activation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids Q589-K652 (specific to isoforms A/B/C) was used as the immunogen for the NFAT2 antibody.
Limitation:
This NFAT2 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O95644