NSJ Bioreagents

HNF6 Antibody

Product Code:
 
NSJ-R31338
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the HNF6 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 5
Western blot testing of HNF6 antibody and Lane 1: rat liver; 2: mouse liver; 3: human HeLa cell lysate. Expected/observed size ~51KD
2 / 5
IHC-P: HNF6 antibody testing of human liver cancer tissue
3 / 5
ICC testing of HNF6 antibody and HCT116 cells
4 / 5
IHC-F testing of rat liver tissue
5 / 5
IHC-P testing of rat liver tissue
❮❯

Western blot testing of HNF6 antibody and Lane 1: rat liver; 2: mouse liver; 3: human HeLa cell lysate. Expected/observed size ~51KD
IHC-P: HNF6 antibody testing of human liver cancer tissue
ICC testing of HNF6 antibody and HCT116 cells
IHC-F testing of rat liver tissue
IHC-P testing of rat liver tissue

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CodeSizePrice
NSJ-R31338-100ug100 ug£535.00
Quantity:
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the HNF6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
One cut homeobox 1 (ONECUT1), also called Hepatocyte nuclear factor 6 (HNF6) is found strong expression in liver and lower expression in testis and skin. The gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human ONECUT1/HNF6 (LGSIHNSQQGLPHYAHP) was used as the immunogen for this HNF6 antibody (100% homologous in human, mouse and rat).
Limitation:
This HNF6 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UBC0