NSJ Bioreagents

HDGF Antibody / Hepatoma Derived Growth Factor

Product Code:
 
NSJ-R32600
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the HDGF antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 7
Western blot testing of 1) rat liver and 2) human 22RV1 (prostate carcinoma) lysate with HDGF antibody at 0.5ug/ml. Predicted molecular weight ~26 kDa but routinely observed at ~38 kDa.
2 / 7
IHC testing of FFPE human lung carcinoma with HDGF antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 7
IHC testing of FFPE human intestinal carcinoma with HDGF antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 7
IHC testing of FFPE mouse liver tissue with HDGF antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
5 / 7
IHC testing of FFPE rat liver tissue with HDGF antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
6 / 7
Immunofluorescent staining of FFPE human U-2 OS cells with HDGF antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 7
Flow cytometry testing of human A431 cells with HDGF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HDGF antibody.
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Western blot testing of 1) rat liver and 2) human 22RV1 (prostate carcinoma) lysate with HDGF antibody at 0.5ug/ml. Predicted molecular weight ~26 kDa but routinely observed at ~38 kDa.
IHC testing of FFPE human lung carcinoma with HDGF antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human intestinal carcinoma with HDGF antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE mouse liver tissue with HDGF antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat liver tissue with HDGF antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with HDGF antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A431 cells with HDGF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HDGF antibody.

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CodeSizePrice
NSJ-R32600-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the HDGF antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Hepatoma-derived growth factor (HDGF), also known as high mobility group protein 1-like 2 (HMG-1L2), is a protein that in humans is encoded by the HDGF gene. This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. And this gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 61-97 (KDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKA-human) were used as the immunogen for the HDGF antibody.
Limitation:
This antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P51858