CUL2 Antibody / Cullin-2

Western blot: 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the CUL2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Cullin-2 is a protein that in humans is encoded by the CUL2 gene. Using immunofluorescence, it was shown that CUL2 is a cytosolic protein that can be translocated to the nucleus by VHL. By fluorescence in situ hybridization, Clifford et al.(1999) mapped the gene to 10p11.2-p11.1, a region reported to show loss of heterozygosity (LOH) in several forms of human cancer, including non-clear cell renal cell carcinoma. Pause et al.(1997) suggested that CUL2 is a candidate tumor suppressor gene, as has been proposed for CUL1 (603134). Lonergan et al.(1998) demonstrated that formation of the VBC-CUL2 complexes is linked to the regulation of hypoxia-inducible mRNAs by VHL. CUL2 is one of several proteins required for degradation of a class of RNA-binding germline proteins in somatic cells of the early blastomere.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

An amino acid sequence from the middle region of human Cullin-2 (HECQQRMVADHLQFLHA) was used as the immunogen for this CUL2 antibody.

This Cullin 2 antibody is available for research use only.

Antigen affinity

Human, Mouse, Rat

Q13617