NSJ Bioreagents

COMT Antibody

Product Code:
 
NSJ-R32010
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the COMT antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 8
Western blot testing of 1) rat brain, 2) rat liver, 3) rat kidney, 4) mouse brain, 5) Jurkat, 6) CEM and 7) HeLa lysate with COMT antibody. Predicted molecular weight: ~30/25 kDa (isoforms 1/2).
2 / 8
IHC testing of FFPE human placenta with COMT antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 8
IHC testing of FFPE mouse lung with COMT antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 8
IHC testing of FFPE rat lung with COMT antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
5 / 8
ICC testing of human A549 cells with COMT antibody.
6 / 8
IHC testing of frozen human placental tissue with COMT antibody.
7 / 8
IHC testing of frozen mouse lung tissue with COMT antibody.
8 / 8
IHC testing of frozen rat lung tissue with COMT antibody.
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Western blot testing of 1) rat brain, 2) rat liver, 3) rat kidney, 4) mouse brain, 5) Jurkat, 6) CEM and 7) HeLa lysate with COMT antibody. Predicted molecular weight: ~30/25 kDa (isoforms 1/2).
IHC testing of FFPE human placenta with COMT antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse lung with COMT antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat lung with COMT antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
ICC testing of human A549 cells with COMT antibody.
IHC testing of frozen human placental tissue with COMT antibody.
IHC testing of frozen mouse lung tissue with COMT antibody.
IHC testing of frozen rat lung tissue with COMT antibody.

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CodeSizePrice
NSJ-R32010-100ug100 ug£535.00
Quantity:
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Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
Optimal dilution of the COMT antibody should be determined by the researcher.
Description:
Catechol O-methyltransferase, also called COMT, is one of the major mammalian enzymes involved in the metabolic degradation of catecholamines. Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. [UniProt]
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 52-271 of human COMT were used as the immunogen for the COMT antibody.
Limitation:
This COMT antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P21964