NSJ Bioreagents

Caspase-6 Antibody

Product Code:
 
NSJ-R30512
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Caspase-6 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE human colorectal adenocarcinoma tissue with Caspase-6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human pancreatic cancer tissue with Caspase-6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human Jurkat cell lysate with Caspase-6 antibody. Predicted molecular weight: ~33 kDa (precursor).
4 / 4
Flow cytometry testing of human SiHa cells with Caspase-6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caspase-6 antibody.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with Caspase-6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic cancer tissue with Caspase-6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human Jurkat cell lysate with Caspase-6 antibody. Predicted molecular weight: ~33 kDa (precursor).
Flow cytometry testing of human SiHa cells with Caspase-6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caspase-6 antibody.

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CodeSizePrice
NSJ-R30512-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the Caspase-6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Caspase-6 is an enzyme that in humans is encoded by the CASP6 gene. This gene encodes a protein that is a member of the cysteine-aspartic acid protease family. Using radiation hybrid mapping, the CASP6 gene was localized to human chromosome 4q25-q26. It functions as a downstream enzyme in the caspase activation cascade, and can cleave Lamin A to its signature apoptotic fragment.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 24-44 (AFYKREMFDPAEKYKMDHRRR-human) were used as the immunogen for this Caspase-6 antibody. This sequence is from the large subunit.
Limitation:
This Caspase-6 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P55212