NSJ Bioreagents

Caspase-4 Antibody

Product Code:
 
NSJ-RQ4240
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Caspase 4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 5
Western blot testing of human 1) HeLa 2) COLO320, 3) SW620 and 4) HepG2 lysate with Caspase 4 antibody at 0.5ug/ml. Predicted molecular weight: ~43 kDa (precursor), ~20 kDa (large subunit).
2 / 5
IHC testing of FFPE human breast cancer tissue with Caspase 4 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
IHC testing of FFPE human colon cancer tissue with Caspase 4 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
IF/ICC stainning of FFPE human A431 cells with Caspase 4 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human A431 cells with Caspase 4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caspase 4 antibody.
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Western blot testing of human 1) HeLa 2) COLO320, 3) SW620 and 4) HepG2 lysate with Caspase 4 antibody at 0.5ug/ml. Predicted molecular weight: ~43 kDa (precursor), ~20 kDa (large subunit).
IHC testing of FFPE human breast cancer tissue with Caspase 4 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human colon cancer tissue with Caspase 4 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IF/ICC stainning of FFPE human A431 cells with Caspase 4 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human A431 cells with Caspase 4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caspase 4 antibody.

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NSJ-RQ4240-100ug100 ug£535.00
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Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence/Immunocytochemistry: 1-3ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Caspase 4 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Caspase 4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of cysteine proteases called caspases. The Caspase 4 gene is mapped to a P1 clone containing the ICE gene, which is located at chromosome 11q22.2-q22.3. It contains 8 coding exons. The function of caspase 4 is not fully known, but it is believed to be an inflammatory caspase, along with caspase 1, caspase 5 (and the murine homolog caspase 11), with a role in the immune system.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids H111-R194 was used as the immunogen for the Caspase 4 antibody. This sequence is from the large subunit.
Limitation:
This Caspase 4 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P49662