NSJ Bioreagents

Caspase-3 Antibody

Product Code:
 
NSJ-R30358
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Caspase-3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of Caspase-3 antibody and Lane 1: HeLa; 2: SMMC-7721 cell lysate
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IHC-P: Caspase-3 antibody testing of human intestinal cancer tissue
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IHC-P: Caspase-3 antibody testing of human lung cancer tissue
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Western blot testing of Caspase-3 antibody and Lane 1: HeLa; 2: SMMC-7721 cell lysate
IHC-P: Caspase-3 antibody testing of human intestinal cancer tissue
IHC-P: Caspase-3 antibody testing of human lung cancer tissue

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NSJ-R30358-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Caspase-3, also known as YAMA, CPP32 or APOPAIN, is a member of the cysteine-aspartic acid protease family known to interact with family members -8 and -9. It is encoded by the CASP3 gene. Tiso et al.(1996) used radiation hybrid mapping to localize the gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al.(1994) found that overexpression of CASP3 in insect cells induced apoptosis. Coexpression of the two CASP3 subunits in insect cells also resulted in apoptosis. Tewari et al.(1995) showed that purified human CASP1 cleaved the CASP3 proenzyme into a proteolytically active form and that activated Caspase-3 cleaved PARP into the 85-kD apoptotic form.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of the human protein (CAMLKQYADKLEFMHIL) was used as the immunogen for this Caspase-3 antibody. This amino acid sequence is from the small subunit.
Limitation:
This Caspase-3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P42574