NSJ Bioreagents

Arginase 1 Antibody

Product Code:
 
NSJ-R30878
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the Arginase 1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of Arginase 1 antibody and Lane 1: rat liver ; 2: rat kidney lysate~
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Western blot testing of 1) rat liver and 2) mouse liver lysate with Arginase 1 antibody. Predicted molecular weight ~35 kDa.
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Western blot testing of Arginase 1 antibody and Lane 1: rat liver ; 2: rat kidney lysate~
Western blot testing of 1) rat liver and 2) mouse liver lysate with Arginase 1 antibody. Predicted molecular weight ~35 kDa.

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NSJ-R30878-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the Arginase 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Arginase is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded by ARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat ARG1 cDNA clone to probe a human liver cDNA library, Haraguchi et al.(1987) isolated and characterized a cDNA corresponding to the gene. By immunologic studies, 90% of the arginase in red blood cells and liver was precipitated by an Arginase 1 antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 307-323 (KREGNHKPETDYLKPPK-rat) were used as the immunogen for this Arginase 1 antibody.
Limitation:
This Arginase 1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
P07824