NSJ Bioreagents

AQP1 Antibody / Aquaporin 1

Product Code:
 
NSJ-R30178
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the AQP1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 5
Western blot testing of AQP1 antibody and Lane 1: rat kidney; 2: rat lung; 3: SMMC-7721 cell lysate
2 / 5
IHC-P: AQP1 antibody testing of human kidney cancer tissue
3 / 5
IHC-P: AQP1 antibody testing of rat kidney tissue
4 / 5
IHC-F testing of AQP1 antibody and rat kidney tissue
5 / 5
Flow cytometry testing of human U-2 OS cells with AQP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=AQP1 antibody.
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Western blot testing of AQP1 antibody and Lane 1: rat kidney; 2: rat lung; 3: SMMC-7721 cell lysate
IHC-P: AQP1 antibody testing of human kidney cancer tissue
IHC-P: AQP1 antibody testing of rat kidney tissue
IHC-F testing of AQP1 antibody and rat kidney tissue
Flow cytometry testing of human U-2 OS cells with AQP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=AQP1 antibody.

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CodeSizePrice
NSJ-R30178-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the AQP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Aquaporin 1 is a 28-kD integral protein though at first to be a breakdown product of the Rh polypeptide but was later shown to be a unique molecule that is abundant in erythrocytes and renal tubules as well as being expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Aquaporin 1 (EEYDLDADDINSRVEMKPK) was used as the immunogen for this AQP1 antibody.
Limitation:
This AQP1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P29972