NSJ Bioreagents

Alkaline Phosphatase Antibody (tissue-nonspecific)

Product Code:
 
NSJ-R30169
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
The lyophilized Alkaline Phosphatase antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of Alkaline Phosphatase antibody at 0.5ug/ml and HT1080 lysate~
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IHC-P: Alkaline Phosphatase antibody testing of rat small intestine tissue
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Western blot testing of Alkaline Phosphatase antibody at 0.5ug/ml and HT1080 lysate~
IHC-P: Alkaline Phosphatase antibody testing of rat small intestine tissue

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CodeSizePrice
NSJ-R30169-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Titration of the Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Alkaline phosphatase, tissue-nonspecific isozyme is an enzyme that in humans is encoded by the ALPL gene. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Liver/bone/kidney alkaline phosphatase, known as tissue-nonspecific, acts physiologically as a lipid-anchored phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) ectophosphatase. Mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 21-35 (EKEKDPKYWRDQAQE) of human ALPL were used as the immunogen for this tissue-nonspecific Alkaline Phosphatase antibody.
Limitation:
This Alkaline Phosphatase antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P05186