NSJ Bioreagents

ADA Antibody / Adenosine deaminase

Product Code:
 
NSJ-R32414
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Adenosine deaminase antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with Adenosine deaminase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC testing of FFPE human tonsil with Adenosine deaminase antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
3 / 4
Western blot testing of human 1) HeLa and 2) COLO320 cell lysate with Adenosine deaminase antibody. Expected molecular weight ~41 kDa.
4 / 4
Flow cytometry testing of human Jurkat cells with Adenosine deaminase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Adenosine deaminase antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Adenosine deaminase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human tonsil with Adenosine deaminase antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Western blot testing of human 1) HeLa and 2) COLO320 cell lysate with Adenosine deaminase antibody. Expected molecular weight ~41 kDa.
Flow cytometry testing of human Jurkat cells with Adenosine deaminase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Adenosine deaminase antibody.

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CodeSizePrice
NSJ-R32414-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Adenosine deaminase antibody should be determined by the researcher.
Description:
Adenosine deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. Primarily, ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids Q135-L363 of the human protein were used as the immunogen for the Adenosine deaminase antibody.
Limitation:
This Adenosine deaminase antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P00813