NSJ Bioreagents

Actin Antibody

Product Code:
 
NSJ-R30001
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
AC-40
Regulatory Status:
 
RUO
Target Species:
  • Chicken (Gallus)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Actin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
 

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CodeSizePrice
NSJ-R30001-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 2ug/ml,Immunohistochemistry (Frozen): 4ug/ml to detect Actin in methanol-fixed tissues
Application Note:
The stated application concentrations are suggested starting points. Titration of the Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
58
Immunogen:
An Actin C-terminal peptide (SGPSIVHRKCF) was used as the immunogen for this Actin antibody.
Limitation:
This Actin antibody is available for research use only.
Purity:
Unpurified ascites
Species Reactivity :
Human, mouse, rat, chicken