Ac-Trp-Leu-Ala-AMC [Ac-WLA-AMC]

South Bay Bio
Product Code: SBB-PS0008
Product Group: Other Biochemicals
Supplier: South Bay Bio

CodeSizePrice
SBB-PS0008-M0022 mg£193.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Human
Shipping:
Blue Ice
Storage:
4C

Images

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Chemical Structure

Chemical Structure

Further Information

Alternate Names/Synonyms:
Proteasome Substrate
Appearance:
Lyophilized powder.
CAS:
1104011-59-7
EClass:
32160000
Form (Short):
solid
Handling Advice:
Avoid freeze/thaw cycles.Protect from light.
InChi:
InChI=1S/C32H37N5O6/c1-17(2)12-26(37-32(42)27(35-20(5)38)14-21-16-33-25-9-7-6-8-24(21)25)31(41)34-19(4)30(40)36-22-10-11-23-18(3)13-29(39)43-28(23)15-22/h6-11,13,15-17,19,26-27,33H,12,14H2,1-5H3,(H,34,41)(H,35,38)(H,36,40)(H,37,42)/t19-,26-,27-/m0/s1
InChiKey:
PIJMRHCPKXLMIS-DOYMOWJKSA-N
Long Description:
Chemical. CAS: 1104011-59-7. Formula: C32H37N5O6. MW: 587.7. Synthetic. Ac-WLA-AMC is a fluorogenic peptid substrate for measuring chymotrypsin-like activity of the proteasome. Hydrolysis of this substrate by the beta5-subunit of the 20S proteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to the constitutive proteasome and is not hydrolyzed efficiently by the immunoproteasome. 20S Proteasome enzyme requires activation with 0.035% SDS in the assay buffer.
Molecular Formula:
C32H37N5O6
Molecular Weight:
587.7
Package Type:
Plastic Vial
Product Description:
Ac-WLA-AMC is a fluorogenic peptid substrate for measuring chymotrypsin-like activity of the proteasome. Hydrolysis of this substrate by the beta5-subunit of the 20S proteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. This substrate is specific to the constitutive proteasome and is not hydrolyzed efficiently by the immunoproteasome. 20S Proteasome enzyme requires activation with 0.035% SDS in the assay buffer.
Purity:
>95% (HPLC)
Sequence:
Acetyl-Trp-Leu-Ala-7-amido-4-methylcoumarin
Solubility Chemicals:
Soluble in DMSO.
Transportation:
Non-hazardous
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.

References

Characterization of a new series of non-covalent proteasome inhibitors with exquisite potency and selectivity for the 20S beta5-subunit: C. Blackburn, et al.; Biochem. J. 430, 461 (2010) | The immunoproteasome as a therapeutic target for hematological malignancies: Z. Miller, et al.; Curr. Can. Drug Targets 14, 537 (2014) | Immunoproteasome-selective inhibitors: a promising strategy to treat hematologic malignancies, autoimmune and inflammatory diseases: R. Ettari, et al.; Curr. Med. Chem. 23, 1217 (2016)