ProSci

POLR2J Antibody

Product Code:
 
PSI-55-770
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).

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PSI-55-770-400ul400ul£626.00
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Further Information

Additional Names:
DNA-directed RNA polymerase II subunit RPB11-a, RNA polymerase II subunit B11-a, RPB11a, DNA-directed RNA polymerase II subunit J-1, RNA polymerase II 133 kDa subunit, POLR2J, POLR2J1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.
Background References:
  • Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
  • Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
  • Shpakovskii, D.G., et al. Bioorg. Khim. 30(6):621-625(2004)
  • Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This POLR2J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the C-terminal region of human POLR2J.
NCBI Gene ID #:
5439
NCBI Official Name:
DNA-directed RNA polymerase II subunit RPB11-a
NCBI Official Symbol:
POLR2J
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
13 kDa
Protein Accession #:
P52435
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P52435
User NOte:
Optimal dilutions for each application to be determined by the researcher.