ProSci

CENPK Antibody

Product Code:
 
PSI-63-891
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of CENPK Antibody in mouse liver tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with CENPK Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of CENPK Antibody in mouse liver tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with CENPK Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-891-400ul400ul£626.00
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Further Information

Additional Names:
Centromere protein K, CENP-K, Interphase centromere complex protein 37, Protein AF-5alpha, p33, CENPK, ICEN37
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression.
Background References:
  • Cheeseman,I.M., et.al., Mol. Biol. Cell 19 (2), 587-594 (2008)
  • Izuta,H., et.al., Genes Cells 11 (6), 673-684 (2006)
  • Okada,M., et.al., Nat. Cell Biol.(5), 446-457 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CENPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-266 amino acids from the C-terminal region of human CENPK.
NCBI Gene ID #:
64105
NCBI Official Name:
Centromere protein K
NCBI Official Symbol:
CENPK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
32 kDa
Protein Accession #:
Q9BS16
Protein GI Number:
74732954
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9BS16
User NOte:
Optimal dilutions for each application to be determined by the researcher.