ProSci

TBX15 Antibody

Product Code:
 
PSI-59-939
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Fluorescent image of HUVEC cell stained with TBX15 Antibody (N-term).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX15 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TBX15 immunoreactivity is localized to Nucleus significantly.
2 / 2
Western blot analysis in mouse liver tissue lysates (35ug/lane).
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Fluorescent image of HUVEC cell stained with TBX15 Antibody (N-term).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX15 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TBX15 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in mouse liver tissue lysates (35ug/lane).

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PSI-59-939-400ul400ul£626.00
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Further Information

Additional Names:
T-box transcription factor TBX15, T-box protein 15, T-box transcription factor TBX14, T-box protein 14, TBX15, TBX14
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
Probable transcriptional regulator involved in developmental processes.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TBX15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-73 amino acids from the N-terminal region of human TBX15.
NCBI Gene ID #:
6913
NCBI Official Name:
T-box transcription factor TBX15
NCBI Official Symbol:
TBX15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
Q96SF7
Protein GI Number:
59803103
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96SF7
User NOte:
Optimal dilutions for each application to be determined by the researcher.