ProSci

DRD2 Antibody

Product Code:
 
PSI-58-687
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: HT-1080 whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution + human brain lysates Lysates/proteins at 20 ug per lane.
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Western Blot at 1:1000 dilution + K562 whole cell lysates Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution + HT-1080 whole cell lysates Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution + human brain lysates Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: HT-1080 whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human brain lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + K562 whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + HT-1080 whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human brain lysates Lysates/proteins at 20 ug per lane.

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PSI-58-687-400ul400ul£626.00
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Further Information

Additional Names:
D(2) dopamine receptor, Dopamine D2 receptor, DRD2
Application Note:
For WB starting dilution is: 1:2000
Background:
This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing.
Background References:
  • Verma, V., et al. J. Biol. Chem. 285(45):35092-35103(2010)
  • Borroto-Escuela, D.O., et al. Biochem. Biophys. Res. Commun. 401(4):605-610(2010)
  • Stelzel, C., et al. J. Neurosci. 30(42):14205-14212(2010)
  • Huang, H.Y., et al. J. Formos. Med. Assoc. 109(10):736-739(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DRD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307-336 amino acids from the C-terminal region of human DRD2.
NCBI Gene ID #:
1813
NCBI Official Name:
D(2) dopamine receptor
NCBI Official Symbol:
DRD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
P14416
Protein GI Number:
118206
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction,Growth Factor
Swissprot #:
P14416
User NOte:
Optimal dilutions for each application to be determined by the researcher.