ProSci

PPID Antibody

Product Code:
 
PSI-63-645
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PPID Antibody in NIH-3T3 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with PPID Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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4 / 4
Western blot analysis of PPID using rabbit polyclonal PPID Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPID gene.
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Western blot analysis of PPID Antibody in NIH-3T3 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with PPID Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PPID using rabbit polyclonal PPID Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPID gene.

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PSI-63-645-400ul400ul£626.00
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Further Information

Additional Names:
Peptidyl-prolyl cis-trans isomerase D, PPIase D, 40 kDa peptidyl-prolyl cis-trans isomerase, Cyclophilin-40, CYP-40, Cyclophilin-related protein, Rotamase D, PPID, CYP40, CYPD
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Background References:
  • Mayya V.,et.al., Sci. Signal. 2:RA46-RA46(2009).
  • Gevaert K., et.al., Nat. Biotechnol. 21:566-569(2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This PPID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-115 amino acids from the N-terminal region of human PPID.
NCBI Gene ID #:
5481
NCBI Official Name:
Peptidyl-prolyl cis-trans isomerase D
NCBI Official Symbol:
PPID
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
41 kDa
Protein Accession #:
Q08752
Protein GI Number:
729274
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology
Swissprot #:
Q08752
User NOte:
Optimal dilutions for each application to be determined by the researcher.