ProSci

MAPK12 Antibody

Product Code:
 
PSI-62-739
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 ug per lane.
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Western blot analysis of p38 gamma (MAPK12) Antibody in mouse lung tissue lysates (35ug/lane)
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Western blot analysis of MAPK12 using rabbit polyclonal hMAPK12-P354 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPK12 gene.
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of p38 gamma (MAPK12) Antibody in mouse lung tissue lysates (35ug/lane)
Western blot analysis of MAPK12 using rabbit polyclonal hMAPK12-P354 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPK12 gene.

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PSI-62-739-400ul400ul£626.00
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Further Information

Additional Names:
Mitogen-activated protein kinase 12, MAP kinase 12, MAPK 12, Extracellular signal-regulated kinase 6, ERK-6, Mitogen-activated protein kinase p38 gamma, MAP kinase p38 gamma, Stress-activated protein kinase 3, MAPK12, ERK6, SAPK3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. MAPK12 functions as a signal transducer during differentiation of myoblasts to myotubes.
Background References:
  • Garcia-Lora, A., et al., Cancer Immunol. Immunother. 52(1):59-64 (2003).
  • Julien, C., et al., J. Biol. Chem. 278(43):42615-42624 (2003).
  • Robinson, M.J., et al., J. Biol. Chem. 277(7):5094-5100 (2002).
  • Court, N.W., et al., J. Mol. Cell. Cardiol. 34(4):413-426 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MAPK12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 339-367 amino acids from the Central region of human MAPK12.
NCBI Gene ID #:
6300
NCBI Official Name:
Mitogen-activated protein kinase 12
NCBI Official Symbol:
MAPK12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
P53778
Protein GI Number:
2851522
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction,
Swissprot #:
P53778
User NOte:
Optimal dilutions for each application to be determined by the researcher.