ProSci

GCLC Antibody

Product Code:
 
PSI-59-915
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

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PSI-59-915-400ul400ul£626.00
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Further Information

Additional Names:
Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GCLC, GLCL, GLCLC
Application Note:
For WB starting dilution is: 1:1000
Background:
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
Background References:
  • Man, B.L., et al.Clin Neurosci 17(10):1244-1247(2010)
  • Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :
  • Le, T.M., et al. Mol. Genet. Metab. 101(1):55-61(2010)
  • Fullerton, J.M., et al. Bipolar Disord 12(5):550-556(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This GCLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human GCLC.
NCBI Gene ID #:
2729
NCBI Official Name:
Glutamate--cysteine ligase catalytic subunit
NCBI Official Symbol:
GCLC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
73 kDa
Protein Accession #:
P48506
Protein GI Number:
1346190
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P48506
User NOte:
Optimal dilutions for each application to be determined by the researcher.