ProSci

CD49e Antibody

Product Code:
 
PSI-61-569
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of CD49e antibody in mouse bladder tissue lysates (35ug/lane)
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Flow cytometric analysis of HepG2 cells using CD49e Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of CD49e antibody in mouse bladder tissue lysates (35ug/lane)
Flow cytometric analysis of HepG2 cells using CD49e Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-569-400ul400ul£626.00
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Further Information

Additional Names:
Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e, Integrin alpha-5 heavy chain, Integrin alpha-5 light chain, ITGA5, FNRA
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
CD49e belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein is the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Background References:
  • Boehmler,A.M., J. Immunol. 182 (11), 6789-6798 (2009)
  • Okazaki,T., Am. J. Pathol. 174 (6), 2378-2387 (2009)
  • Schornberg,K.L., Proc. Natl. Acad. Sci. U.S.A. 106 (19), 8003-8008 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CD49e antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 796-824 amino acids from the C-terminal region of human CD49e.
NCBI Gene ID #:
3678
NCBI Official Name:
Integrin alpha-5
NCBI Official Symbol:
ITGA5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
115 kDa
Protein Accession #:
P08648
Protein GI Number:
23831237
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
P08648
User NOte:
Optimal dilutions for each application to be determined by the researcher.