ProSci

GC Antibody

Product Code:
 
PSI-63-709
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of GC Antibody in mouse lung tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human Skin reacted with GC Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of GC Antibody in mouse lung tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human Skin reacted with GC Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-709-200ul200ul£626.00
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Further Information

Additional Names:
Glucosylceramidase, Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, Beta-GC, D-glucosyl-N-acylsphingosine glucohydrolase, Imiglucerase, GBA, GC, GLUC
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
GC is a protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism.
Background References:
  • Jamrozik,Z., et.al., J. Neurol. 257 (3), 459-460 (2010)
  • Mao,X.Y., et.al., Neurosci. Lett. 469 (2), 256-259 (2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig, Bovine
Immunogen:
This GC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337-365 amino acids from the Central region of human GC.
NCBI Gene ID #:
2629
NCBI Official Name:
Glucosylceramidase
NCBI Official Symbol:
GBA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
P04062
Protein GI Number:
55584151
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Neuroscience,Signal Transduction
Swissprot #:
P04062
User NOte:
Optimal dilutions for each application to be determined by the researcher.