ProSci

PKC gamma Antibody

Product Code:
 
PSI-62-595
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-PKC gamma C-term Pab in mouse brain lysate. PKC gamma was detected using purified Pab.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC gamma antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-PKC gamma C-term Pab in mouse brain lysate. PKC gamma was detected using purified Pab.
Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC gamma antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-595-400ul400ul£626.00
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Further Information

Additional Names:
Protein kinase C gamma type, PKC-gamma, PRKCG, PKCG
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).
Background References:
  • Uchino, M., et al., J. Biol. Chem. 279(3):2254-2261 (2004).
  • van de Warrenburg, B.P., et al., Neurology 61(12):1760-1765 (2003).
  • Yabe, I., et al., Arch. Neurol. 60(12):1749-1751 (2003).
  • Ananthanarayanan, B., et al., J. Biol. Chem. 278(47):46886-46894 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This PKC gamma antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-336 amino acids from the C-terminal region of human PKC gamma.
NCBI Gene ID #:
5582
NCBI Official Name:
Protein kinase C gamma type
NCBI Official Symbol:
PRKCG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
78 kDa
Protein Accession #:
P05129
Protein GI Number:
462455
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Neuroscience,Signal Transduction
Swissprot #:
P05129
User NOte:
Optimal dilutions for each application to be determined by the researcher.