ProSci

GDF10 Antibody

Product Code:
 
PSI-61-064
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of GDF10 Antibody in NIH-3T3 cell line lysates (35ug/lane)
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Flow cytometric analysis of K562 cells using GDF10 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of GDF10 Antibody in NIH-3T3 cell line lysates (35ug/lane)
Flow cytometric analysis of K562 cells using GDF10 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-61-064-400ul400ul£626.00
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Further Information

Additional Names:
Bone morphogenetic protein 3B, BMP-3B, Bone-inducing protein, BIP, Growth/differentiation factor 10, GDF-10, GDF10, BMP3B
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
GDF10 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that the protein encoded by this gene plays a role in skeletal morphogenesis.
Background References:
  • Ducy, P., et al., Kidney Int. 57(6):2207-2214 (2000).
  • Hino, J., et al., Biochem. Biophys. Res. Commun. 223(2):304-310 (1996).
  • Cunningham, N.S., et al., Growth Factors 12(2):99-109 (1995).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This GDF10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-344 amino acids from the Central region of human GDF10.
NCBI Gene ID #:
2662
NCBI Official Name:
Bone morphogenetic protein 3B
NCBI Official Symbol:
GDF10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
P55107
Protein GI Number:
1705471
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P55107
User NOte:
Optimal dilutions for each application to be determined by the researcher.