ProSci

CAMK2D Antibody

Product Code:
 
PSI-62-734
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Antibody is used in Western blot to detect CAMK2 delta in serum-starved HeLa cell lysate.
2 / 3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
3 / 3
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Antibody is used in Western blot to detect CAMK2 delta in serum-starved HeLa cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

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PSI-62-734-400ul400ul£626.00
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Further Information

Additional Names:
Calcium/calmodulin-dependent protein kinase type II subunit delta, CaM kinase II subunit delta, CaMK-II subunit delta, CAMK2D, CAMKD
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
CaM-kinase II (CAMK2) is a prominent Ser/Thr protein kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Likely autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state. CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Expression of CAMK2 delta is significantly increased in patients suffering from dilated cardiomyopathy.
Background References:
  • Rochlitz, H., et al., Diabetologia 43(4):465-473 (2000).
  • Hoch, B., et al., Circ. Res. 84(6):713-721 (1999).
  • Tombes, R.M., et al., Biochim. Biophys. Acta 1355(3):281-292 (1997).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rabbit, Pig
Immunogen:
This CAMK2D (CAMK2 delta) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 448-478 amino acids from the C-terminal region of human CAMK2D (CAMK2 delta).
NCBI Gene ID #:
817
NCBI Official Name:
Calcium/calmodulin-dependent protein kinase type II subunit delta
NCBI Official Symbol:
CAMK2D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
Protein Accession #:
Q13557
Protein GI Number:
116242602
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Neuroscience,Signal Transduction
Swissprot #:
Q13557
User NOte:
Optimal dilutions for each application to be determined by the researcher.