ProSci

CUX1 Antibody

Product Code:
 
PSI-62-095
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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Fluorescent confocal image of Hela cell stained with CUX1 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CUX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CUX1 immunoreactivity is localized to Nucleus significantly.
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Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Fluorescent confocal image of Hela cell stained with CUX1 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CUX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CUX1 immunoreactivity is localized to Nucleus significantly.

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PSI-62-095-400ul400ul£626.00
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Further Information

Additional Names:
Homeobox protein cut-like 1, CCAAT displacement protein, CDP, Homeobox protein cux-1, CUX1, CUTL1
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CUX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1347-1374 amino acids from the C-terminal region of human CUX1.
NCBI Gene ID #:
1523
NCBI Official Name:
Homeobox protein cut-like 1
NCBI Official Symbol:
CUX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
164 kDa
Protein Accession #:
P39880
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P39880
User NOte:
Optimal dilutions for each application to be determined by the researcher.