ProSci

JHDM1D Antibody

Product Code:
 
PSI-61-796
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of JHDM1D Antibody in 293 cell line lysates (35ug/lane)
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JHDM1D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of JHDM1D Antibody in 293 cell line lysates (35ug/lane)
JHDM1D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-61-796-400ul400ul£626.00
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Further Information

Additional Names:
Lysine-specific demethylase 7A, 11411-, JmjC domain-containing histone demethylation protein 1D, Lysine-specific demethylase 7, KDM7A, JHDM1D, KDM7, KIAA1718
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
JHDM1D histone demethylase that specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3, thereby playing a central role in histone code. This specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. This demethylates H3K9me2 in absence of H3K4me3.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This JHDM1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-316 amino acids from the Central region of human JHDM1D.
NCBI Gene ID #:
80853
NCBI Official Name:
Lysine-specific demethylase 7A
NCBI Official Symbol:
KDM7A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
107 kDa
Protein Accession #:
Q6ZMT4
Protein GI Number:
90111764
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q6ZMT4
User NOte:
Optimal dilutions for each application to be determined by the researcher.