ProSci

RT25 Antibody

Product Code:
 
PSI-64-146
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of RT25 Antibody in MCF-7 cell line lysates (35ug/lane)
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RT25 Antibody IHC analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of RT25 Antibody in MCF-7 cell line lysates (35ug/lane)
RT25 Antibody IHC analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-64-146-400ul400ul£626.00
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Further Information

Additional Names:
28S ribosomal protein S25, mitochondrial, MRP-S25, S25mt, MRPS25, RPMS25
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.
Background References:
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
  • Zhang, Z., et al. Genomics 81(5):468-480(2003)
  • Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :
  • Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids from the C-terminal region of human RT25.
NCBI Gene ID #:
64432
NCBI Official Name:
28S ribosomal protein S25, mitochondrial
NCBI Official Symbol:
MRPS25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
P82663
Protein GI Number:
13633894
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P82663
User NOte:
Optimal dilutions for each application to be determined by the researcher.