ProSci

AARS2 Antibody

Product Code:
 
PSI-62-990
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in K562 cell line lysates (35ug/lane).
2 / 3
AARS2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western blot analysis in K562 cell line lysates (35ug/lane).
AARS2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-62-990-400ul400ul£626.00
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Further Information

Additional Names:
Alanine--tRNA ligase, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03133}, 6117 {ECO:0000255|HAMAP-Rule:MF_03133}, Alanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_03133}, AlaRS {ECO:0000255|HAMAP-Rule:MF_03133}, AARS2 {ECO:0000255|HAMAP-Rule:MF_03133}
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Alanyl-tRNA synthetase 2 (AARS2) is an enzyme that catalyzes the chemical reaction: ATP + L-alanine + tRNAAla <---> AMP + diphosphate + L-alanyl-tRNAAla. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis.
Background References:
  • Bonnefond,L.,Biochemistry 44 (12), 4805-4816 (2005)
  • Nakayama,M., Genome Res. 12 (11), 1773-1784 (2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-394 amino acids from the Central region of human AARS2.
NCBI Gene ID #:
57505
NCBI Official Name:
Alanine--tRNA ligase, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03133}
NCBI Official Symbol:
AARS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
107 kDa
Protein Accession #:
Q5JTZ9
Protein GI Number:
74742244
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q5JTZ9
User NOte:
Optimal dilutions for each application to be determined by the researcher.