ProSci

POU2F1 Antibody

Product Code:
 
PSI-55-997
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis in HL-60 cell line lysates (35ug/lane).
2 / 2
POU2F1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
POU2F1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-997-400ul400ul£626.00
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Further Information

Additional Names:
POU domain, class 2, transcription factor 1, NF-A1, Octamer-binding protein 1, Oct-1, Octamer-binding transcription factor 1, OTF-1, POU2F1, OCT1, OTF1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.
Background References:
  • Vuzman, D., et al. Biophys. J. 99(4):1202-1211(2010)
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Davila, S., et al. Genes Immun. 11(3):232-238(2010)
  • Laurent, B., et al. Blood 115(3):687-695(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Pig, Rat, Xenopus
Immunogen:
This POU2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-391 amino acids from the Central region of human POU2F1.
NCBI Gene ID #:
5451
NCBI Official Name:
POU domain, class 2, transcription factor 1
NCBI Official Symbol:
POU2F1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
76 kDa
Protein Accession #:
P14859
Protein GI Number:
28202257
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P14859
User NOte:
Optimal dilutions for each application to be determined by the researcher.