Further Information
V-type proton ATPase 21 kDa proteolipid subunit, V-ATPase 21 kDa proteolipid subunit, Vacuolar proton pump 21 kDa proteolipid subunit, hATPL, ATP6V0B, ATP6F
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
This gene encodes a component of vacuolar ATPase
(V-ATPase), a multisubunit enzyme that mediates acidification of
eukaryotic intracellular organelles. V-ATPase dependent organelle
acidification is necessary for such intracellular processes as
protein sorting, zymogen activation, receptor-mediated endocytosis,
and synaptic vesicle proton gradient generation. V-ATPase is
composed of a cytosolic V1 domain and a transmembrane V0 domain.
The V1 domain consists of three A and three B subunits, two G
subunits plus the C, D, E, F, and H subunits. The V1 domain
contains the ATP catalytic site. The V0 domain consists of five
different subunits: a, c, c', c'', and d. Additional isoforms of
many of the V1 and V0 subunit proteins are encoded by multiple
genes or alternatively spliced transcript variants. This encoded
protein is part of the transmembrane V0 domain and is the human
counterpart of yeast VMA16. Two alternatively spliced transcript
variants that encode different proteins have been found for this
gene.
- Lu, M., et al. J. Biol. Chem. 282(34):24495-24503(2007)
- Rojas, J.D., et al. Biochem. Biophys. Res. Commun. 320(4):1123-1132(2004)
- Morel, N. Biol. Cell 95(7):453-457(2003)
- Smith, A.N., et al. Mol. Cell 12(4):801-803(2003)
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine
This ATP6V0B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the Central region of human ATP6V0B.
533
V-type proton ATPase 21 kDa proteolipid subunit
ATP6V0B
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Q99437
6136172
This antibody is purified through a protein A column, followed by peptide affinity purification.
Q99437
Optimal dilutions for each application to be determined by the researcher.