ProSci

PAX1 Antibody

Product Code:
 
PSI-55-189
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in HepG2,MDA-MB453,293,K562,MCF-7 cell line lysates (35ug/lane).
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HepG2,MDA-MB453,293,K562,MCF-7 cell line lysates (35ug/lane).

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PSI-55-189-400ul400ul£626.00
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Further Information

Additional Names:
Paired box protein Pax-1, HuP48, PAX1, HUP48
Application Note:
For FACS starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
The PAX genes, including PAX1, are a highly conserved family of developmental control genes that encode transcription factors and have been shown to play a role in pattern formation during embryogenesis in vertebrates (McGaughran et al., 2003 [PubMed 12774041]). See PAX7 (MIM 167410) for a discussion of paired box domain genes.
Background References:
  • Huang, T.H., et al. Int. J. Gynecol. Cancer 20(4):513-519(2010)
  • Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
  • Hillmer, A.M., et al. Nat. Genet. 40(11):1279-1281(2008)
  • Richards, J.B., et al. Nat. Genet. 40(11):1282-1284(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PAX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-471 amino acids from the C-terminal region of human PAX1.
NCBI Gene ID #:
5075
NCBI Official Name:
Paired box protein Pax-1
NCBI Official Symbol:
PAX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
P15863
Protein GI Number:
296439493
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P15863
User NOte:
Optimal dilutions for each application to be determined by the researcher.