ProSci

POLR1E Antibody

Product Code:
 
PSI-58-944
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in K562 cell line lysates (35ug/lane).
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Western blot analysis in K562 cell line lysates (35ug/lane).

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PSI-58-944-400ul400ul£626.00
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Further Information

Additional Names:
DNA-directed RNA polymerase I subunit RPA49, RNA polymerase I subunit A49, DNA-directed RNA polymerase I subunit E, RNA polymerase I-associated factor 1, RNA polymerase I-associated factor 53, POLR1E, PAF53, PRAF1
Application Note:
For WB starting dilution is: 1:1000
Background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity).
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
  • Percipalle, P., et al. EMBO Rep. 7(5):525-530(2006)
  • Yamamoto, K., et al. Mol. Cell. Biol. 24(14):6338-6349(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This POLR1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-198 amino acids from the Central region of human POLR1E.
NCBI Gene ID #:
64425
NCBI Official Name:
DNA-directed RNA polymerase I subunit RPA49
NCBI Official Symbol:
POLR1E
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
Q9GZS1
Protein GI Number:
62901107
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9GZS1
User NOte:
Optimal dilutions for each application to be determined by the researcher.