ProSci

VPS26A Antibody

Product Code:
 
PSI-63-613
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of VPS26A Antibody in HepG2 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with VPS26A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of VPS26A Antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with VPS26A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-613-400ul400ul£626.00
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Further Information

Additional Names:
Vacuolar protein sorting-associated protein 26A, Vesicle protein sorting 26A, hVPS26, VPS26A, VPS26
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
VPS26A is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
Background References:
  • Haft,C.R., et.al., Mol. Biol. Cell 11 (12), 4105-4116 (2000)
  • Reddy,J.V.et.al., Mol. Biol. Cell 12 (10), 3242-3256 (2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Zebrafish, Mouse, Rat, C. Elegans
Immunogen:
This VPS26A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-291 amino acids from the Central region of human VPS26A.
NCBI Gene ID #:
9559
NCBI Official Name:
Vacuolar protein sorting-associated protein 26A
NCBI Official Symbol:
VPS26A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
O75436
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
O75436
User NOte:
Optimal dilutions for each application to be determined by the researcher.