ProSci

CSNK1A1 Antibody

Product Code:
 
PSI-62-883
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CK1a antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-CK1a Pab in A375 cell lysate
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CK1a antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-CK1a Pab in A375 cell lysate

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PSI-62-883-400ul400ul£626.00
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Further Information

Additional Names:
Casein kinase I isoform alpha, CKI-alpha, CK1, CSNK1A1
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CK1a can phosphorylate a large number of proteins. This cytoplasmic protein participates in Wnt signaling. It has been demonstrated to phosphorylate CTNNB1 on Ser-45 and to interact with the Axin complex.
Background References:
  • Liu, C., et al., Cell 108(6):837-847 (2002).
  • Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
  • Fish, K.J., et al., J. Biol. Chem. 270(25):14875-14883 (1995).
  • Tapia, C., et al., FEBS Lett. 349(2):307-312 (1994).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Xenopus
Immunogen:
This CSNK1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-337 amino acids from the C-terminal region of human CSNK1A1.
NCBI Gene ID #:
1452
NCBI Official Name:
Casein kinase I isoform alpha
NCBI Official Symbol:
CSNK1A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
P48729
Protein GI Number:
31077177
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P48729
User NOte:
Optimal dilutions for each application to be determined by the researcher.