ProSci

GKAP1 Antibody

Product Code:
 
PSI-61-941
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
2 / 3
GKAP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
GKAP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-61-941-400ul400ul£626.00
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Further Information

Additional Names:
G kinase-anchoring protein 1, cGMP-dependent protein kinase-anchoring protein of 42 kDa, GKAP1, GKAP42
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
GKAP1 is a protein that is highly similar to the mouse cGMP-dependent protein kinase anchoring protein 42kDa. The mouse protein has been found to localize with the Golgi and recruit cGMP-dependent protein kinase I alpha to the Golgi in mouse testes. It is thought to play a role in germ cell development. Transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Lim, J., et al. Cell 125(4):801-814(2006)
  • Sweetser, D.A., et al. Genes Chromosomes Cancer 44(3):279-291(2005)
  • Yuasa, K., et al. J. Biol. Chem. 275(7):4897-4905(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This GKAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-359 amino acids from the C-terminal region of human GKAP1.
NCBI Gene ID #:
80318
NCBI Official Name:
G kinase-anchoring protein 1
NCBI Official Symbol:
GKAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
Q5VSY0
Protein GI Number:
166224631
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q5VSY0
User NOte:
Optimal dilutions for each application to be determined by the researcher.