ProSci

STUB1 Antibody

Product Code:
 
PSI-56-259
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 3
Western blot analysis in 293 cell line lysates (35ug/lane).
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Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in 293 cell line lysates (35ug/lane).

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PSI-56-259-400ul400ul£626.00
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Further Information

Additional Names:
E3 ubiquitin-protein ligase CHIP, 632-, Antigen NY-CO-7, CLL-associated antigen KW-8, Carboxy terminus of Hsp70-interacting protein, STIP1 homology and U box-containing protein 1 {ECO:0000312|HGNC:HGNC:11427}, STUB1 (HGNC:11427)
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation (Min et al., 2008 [PubMed 18411298]).
Background References:
  • Stankiewicz, M., et al. FEBS J. 277(16):3353-3367(2010)
  • Naito, A.T., et al. Circ. Res. 106(11):1692-1702(2010)
  • Loffek, S., et al. Hum. Mutat. 31(4):466-476(2010)
  • Graf, C., et al. Biochemistry 49(10):2121-2129(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse
Immunogen:
This STUB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-263 amino acids from the C-terminal region of human STUB1.
NCBI Gene ID #:
10273
NCBI Official Name:
E3 ubiquitin-protein ligase CHIP
NCBI Official Symbol:
STUB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
Q9UNE7
Protein GI Number:
78099173
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9UNE7
User NOte:
Optimal dilutions for each application to be determined by the researcher.