ProSci

XYLT1 Antibody

Product Code:
 
PSI-56-641
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-56-641-400ul400ul£626.00
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Further Information

Additional Names:
Xylosyltransferase 1, Peptide O-xylosyltransferase 1, Xylosyltransferase I, XT-I, XylT-I, XYLT1, XT1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This locus encodes a xylosyltransferase enzyme. The encoded protein catalyzes transfer of UDP-xylose to serine residues of an acceptor protein substrate. This transfer reaction is necessary for biosynthesis of glycosaminoglycan chains. Mutations in this gene have been associated with increased severity of pseudoxanthoma elasticum.
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Muller, B., et al. J. Biol. Chem. 284(45):30775-30782(2009)
  • Ponighaus, C., et al. Am. J. Hypertens. 22(4):432-436(2009)
  • Ambrosius, M., et al. Clin. Biochem. 42 (1-2), 1-4 (2009) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This XYLT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the N-terminal region of human XYLT1.
NCBI Gene ID #:
64131
NCBI Official Name:
Xylosyltransferase 1
NCBI Official Symbol:
XYLT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
108 kDa
Protein Accession #:
Q86Y38
Protein GI Number:
71164803
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q86Y38
User NOte:
Optimal dilutions for each application to be determined by the researcher.