ProSci

ARF5 Antibody

Product Code:
 
PSI-58-942
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis in Ramos cell line lysates (35ug/lane).
2 / 2
Western blot analysis of ARF5 using rabbit polyclonal ARF5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARF5 gene.
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Western blot analysis in Ramos cell line lysates (35ug/lane).
Western blot analysis of ARF5 using rabbit polyclonal ARF5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARF5 gene.

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PSI-58-942-400ul400ul£626.00
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Further Information

Additional Names:
ADP-ribosylation factor 5, ARF5
Application Note:
For WB starting dilution is: 1:1000
Background:
ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq].
Background References:
  • Shiba, T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(42):15416-15421(2006)
  • Austin, C., et al. Biochemistry 41(14):4669-4677(2002)
  • Shin, O.H., et al. Biochemistry 40(36):10846-10852(2001)
  • Nevrivy, D.J., et al. J. Biol. Chem. 275(22):16827-16836(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This ARF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-108 amino acids from the Central region of human ARF5.
NCBI Gene ID #:
381
NCBI Official Name:
ADP-ribosylation factor 5
NCBI Official Symbol:
ARF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Protein Accession #:
P84085
Protein GI Number:
51316990
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P84085
User NOte:
Optimal dilutions for each application to be determined by the researcher.