ProSci

AKR1B1 Antibody

Product Code:
 
PSI-61-466
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:8000 dilution Lane 1: A431 whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of anti-AKR1B1 Pab in Jurkat cell line lysates (35ug/lane)
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Confocal immunofluorescent analysis of AKR1B1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western Blot at 1:8000 dilution Lane 1: A431 whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-AKR1B1 Pab in Jurkat cell line lysates (35ug/lane)
Confocal immunofluorescent analysis of AKR1B1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-61-466-400ul400ul£626.00
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Further Information

Additional Names:
Aldose reductase, AR, Aldehyde reductase, Aldo-keto reductase family 1 member B1, AKR1B1, ALDR1
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
AKR1B1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.
Background References:
  • Steuber,H.,J. Mol. Biol. 379 (5), 991-1016 (2008)
  • Gleissner,C.A.,Arterioscler. Thromb. Vasc. Biol. 28 (6), 1137-1143 (2008)
  • Grundmann,U.,DNA Cell Biol.(3), 149-157 (1990)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AKR1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-135 amino acids from the Central region of human AKR1B1.
NCBI Gene ID #:
231
NCBI Official Name:
Aldose reductase
NCBI Official Symbol:
AKR1B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
P15121
Protein GI Number:
113596
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
P15121
User NOte:
Optimal dilutions for each application to be determined by the researcher.