ProSci

NDUFAF1 Antibody

Product Code:
 
PSI-55-386
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
2 / 2
NDUFAF1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
NDUFAF1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-386-400ul400ul£626.00
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Further Information

Additional Names:
Complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1, NDUFAF1, CIA30
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The NDUFAF1 gene encodes the human homolog of a Neurospora crassa Cia30 protein involved in the assembly of complex I.
Background References:
  • Wheeler, H.E., et al. PLoS Genet.(10), E1000685 (2009) :
  • Dunning, C.J., et al. EMBO J. 26(13):3227-3237(2007)
  • Vogel, R.O., et al. Mol. Genet. Metab. 91(2):176-182(2007)
  • Vogel, R.O., et al. Genes Dev. 21(5):615-624(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NDUFAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-276 amino acids from the C-terminal region of human NDUFAF1.
NCBI Gene ID #:
51103
NCBI Official Name:
Complex I intermediate-associated protein 30, mitochondrial
NCBI Official Symbol:
NDUFAF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
Q9Y375
Protein GI Number:
21542405
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q9Y375
User NOte:
Optimal dilutions for each application to be determined by the researcher.