ProSci

Methionine Aminopeptidase 2 Antibody

Product Code:
 
PSI-61-370
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of METAP2 Antibody in 293 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with METAP2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of METAP2 Antibody in 293 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with METAP2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-61-370-400ul400ul£626.00
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Further Information

Additional Names:
Methionine aminopeptidase 2 {ECO:0000255|HAMAP-Rule:MF_03175}, MAP 2 {ECO:0000255|HAMAP-Rule:MF_03175}, MetAP 2 {ECO:0000255|HAMAP-Rule:MF_03175}, 341118 {ECO:0000255|HAMAP-Rule:MF_03175}, Initiation factor 2-associated 67 kDa glycoprotein {ECO:0000255|HAMAP-Rule:MF_03175}, p67 {ECO:0000255|HAMAP-Rule:MF_03175}, p67eIF2 {ECO:0000255|HAMAP-Rule:MF_03175}, Peptidase M {ECO:0000255|HAMAP-Rule:MF_03175}, METAP2 {ECO:0000255|HAMAP-Rule:MF_03175}
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The ADP-ribosylation factor (Arf) family are highly conserved members of the Ras superfamily of regulatory GTP-binding proteins. Arf proteins participate in routing of intracellular proteins to and within the Golgi complex. Cellular functions include maintenance of organelle integrity, coat protein assembly, as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization.
Background References:
  • Wang, J., et al., Biochemistry 42(17):5035-5042 (2003).
  • Datta, R., et al., Exp. Cell Res. 283(2):237-246 (2003).
  • Endo, H., et al., J. Biol. Chem. 277(29):26396-26402 (2002).
  • Kanno, T., et al., Lab. Invest. 82(7):893-901 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This Methionine Aminopeptidase 2 (METAP2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-467 amino acids from the C-terminal region of human Methionine Aminopeptidase 2 (METAP2).
NCBI Gene ID #:
10988
NCBI Official Name:
Methionine aminopeptidase 2 {ECO:0000255|HAMAP-Rule:MF_03175}
NCBI Official Symbol:
METAP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
P50579
Protein GI Number:
1703273
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
P50579
User NOte:
Optimal dilutions for each application to be determined by the researcher.