ProSci

HIBCH Antibody

Product Code:
 
PSI-62-907
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of HIBCH Antibody in K562 cell line lysates (35ug/lane)(2ug/ml)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIBCH antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of HIBCH Antibody in K562 cell line lysates (35ug/lane)(2ug/ml)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIBCH antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-907-400ul400ul£626.00
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Further Information

Additional Names:
3-hydroxyisobutyryl-CoA hydrolase, mitochondrial, 3-hydroxyisobutyryl-coenzyme A hydrolase, HIB-CoA hydrolase, HIBYL-CoA-H, HIBCH
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
HIBCH is responsible for the specific hydrolysis of HIBYL-CoA, a valine catabolite, as well as the hydrolysis of beta-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism.
Background References:
  • Hawes J.W., Jaskiewicz J., Shimomura Y.J. Biol. Chem. 271:26430-26434(1996)
  • Loupatty F.J., Clayton P.T.Am. J. Hum. Genet. 80:195-199(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HIBCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-209 amino acids from the Central region of human HIBCH.
NCBI Gene ID #:
26275
NCBI Official Name:
3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
NCBI Official Symbol:
HIBCH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
Q6NVY1
Protein GI Number:
146324905
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q6NVY1
User NOte:
Optimal dilutions for each application to be determined by the researcher.